???????DPV?????????Duck Enter-itis Virus,DEV?????????α-???????????????????????????????????????????1????????? ? ? ? ??? ? ? ?160~180 nm,? ? ??core?????capsid?????tegument?????enve-lope???????2??????????????TK?????????Thymidine Kinase,TK??????????????---?????????????????TK?????????????????
??1 TK??
????????????DNA,?150kb,???????????????????????CHv?????????C-KCE????????VAC?????????UL?Unique Long??US?Unique Short????????????UL1?UL2?UL3??US1?US2?US3????TK????UL23,??TK????UL23???
??TK??????????????????VAC?????74 481~75 557?????CHv????77 994~79 070? ??? ?1 077bp,? ? ? ????3??
???DPVTK???α?β?γ?????TK?????????????????DPV?α?????????36.8%????β?26.8%??γ?????29.6%?????α??????????????Marek's Disease Virus,MDV??? ??36.3%?????????????Avian InfectiousLaryngotracheitis Virus,ILTV?????36.8%?????????Herpevirus of turkey,HVT?????40.6%????????????????4??
?????????????????????TK????????????????GD?TK?? ? ? ? ?DPVclone-03??? ? ? ? ? ?AV1221???Chv????????????????99.9%????????????? ?YZH?TK????GenBank????????????????5??????TK??????????99%.??????????????AV1221?TK???EF173464??????????? ?TK? ? ?AY911509?? ? ? ? ? ? ?99.4%,??????????????????TK???????5~8???????????????????????????????TK????????????????????????????????????????????????????????
??2 TK??
??TK????358??????????????????????????43???????47???????130???????83??
??TK???????????????????????????????????????????TK?????????α-???β-???????????????48.9%?7.3%,????16%?????????????????????????TK?????????????????
??5???????2?N-??????1?ATP/GTP?????3?????????1????????????????????????????????9??
??TK?????????????????????????????????????????dTMP,??????dTTP???DNA????
??TK????????????????????????2???????N??ATP?????-DGXXGXGK-????????-DRH-,???????31~39??133~135????ATP??????TK?????????????????????????ATP??????????D?Asp??G?Gly??K?Lys???????????????3?G????????????????????G????????TK????????ATP???????TK??????????????????????????????????????????????????????????????????????????????????????????9??
??3 TK???????????
??TK??????????????????????????????????????????????????TK???????????????????????????????????????????????????????TK?????????????????????????TK????????????
???????????UL24?????TK???????????TK?????UL22????????????TK????468??????2?LoxP???????EGFP?????????? ? ? ? ? ? ? ?pSKLR-EGEP-LoxP,? ?DEF????????EGFP????10???????????????C-KCE??????TK??ORF?2.7kb?????TK???244bp?????lacZ????????????pTK-LacZ,?????TK??????Pcmv-GFP-SV40pA?????????pTK-GFP?11~12?????????????TK????????????
??????TK????????????????????????????????????????????????????????????I??????VP1?????????TK????? ? ? ? ? ? ? ? ? ? ?pBlueSK-TK-EGFP-VP1,??DEF????????????????????????54kDa????????VP1????????????I??????3D??????pBlueSK-TK-EGFP-3D,?????????GD01?????????13~14?????????TK??1 059bp????????????487bp,????471bp???????????EGFP????H5N1??HA?NA?????pGTK-EGFP-HA-NA,??CEF??????????????????????????????????EGFP-HA-NA?15????????DPV34F2?TK?????????lacZ????HA?????????????PCR???????1 700bp?HA???????Westernblot??????70kDa??????16??????????????????????????????????????????????????????????????????????????????TK??????????????????HA?NA?????????????????????????????????????????????????????????????????????
??4??
??TK????????????????TK?????????????????????????????????????????????????????TK??????????????????????????????????????TK?gE??????????????????????????????????????????????????????????????????????????TK??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
??????
???1?????????????????????????J?????????2014?7??16~19.
???2??????????????????????????????J?????????2003,24?6??25~28.
???3?Li Y1,Huang B,Ma X,et al.Molecular characterizationof the genome of duck enteritis virus?J??Virology,2009,391?2??151~161.
???4?????????????????TK???????????? ? ?J??? ? ? ? ? ??2008,38?04??297~302.
???5???????????????????GD?TK?dUT-Pase?PK???????????J?????????2010,31?5??53~57.
???6??????????????????YZH????????TK????????J?????????2012,33?12??133~137.
???7??????????????????AV1221?TK???????????J???????????2007,24?3??162~164.